![]() ![]() ![]() ![]() Conversely, angiotensin II increased cell numbers up to 4-fold while retaining cell viability. Irbesartan reduced cell numbers by 50% and induced morphologic changes with loss of pseudopods ( P < 0.05). Levels of ROS were almost completely attenuated by irbesartan (DHE fluorescence intensity of 5.68E-09) ( P < 0.05). Irbesartan inhibited HTF migration by 50% to 70% compared to controls ( P < 0.05). To assess the effect on reactive oxygen species (ROS) level, HTF were treated with either irbesartan (10 μg/mL) or angiotensin II (2 μg/mL) for 24 hours after scratching, and then stained with dihydroethidium (DHE) before evaluation by confocal microscopy. The extent of HTF migration up to 30 hours, and cell number and morphology at 72 hours was evaluated. We investigate the effect of angiotensin receptor blockade on the migration of human Tenon fibroblasts (HTF), using irbesartan, an angiotensin II receptor type 1 (AT1R) blocker (ARB) as a potential antifibrotic agent in glaucoma filtration surgery.Ĭonfluent HTF cultures were scratched with a 1 mL pipette tip and treated with either irbesartan (10, 50, and 100 μg/mL) or angiotensin II (2 μg/mL). ![]()
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